400 308-nm excimer lamp ameliorates MC-903 induced atopic dermatitis with a reduction of thymic stromal lymphopoietin mRNA levels
نویسندگان
چکیده
Purpose Narrow band-ultraviolet B (NB-UVB) is efficacious for atopic dermatitis (AD). However, its detailed mechanism remains to be elucidated. The purpose of this study explore an in vivo-effect NB-UVB on AD, utilizing MC903-induced AD model mice. Method To induce AD-like dermatitis, MC903, a vitamin D3 analogue, was applied the shaved dorsal skin C57BL/6 mice once day twelve days. Mice were irradiated with 308-nm excimer lamp at 100 mJ/cm2 every other from days 0 10. Clinical severity evaluated 0-12. Epidermal thickness and infiltrating cells assessed 12, respectively. Real time polymerase chain reaction performed assess mRNA levels cytokines lesions 4,7 12. Furthermore, moisture level pH Results Irradiation alleviated clinically 4 pathologically reduced epidermal number layers Foxp3-positive into increased 12 MC903-applied compared those without irradiation, although significant increase IL-10 not observed In addition, irradiation expression thymic stromal lymphopoietin (TSLP) 4. almost same as irradiation. Conclusions ameliorated reduction TSLP levels.
منابع مشابه
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2022
ISSN: ['1523-1747', '0022-202X']
DOI: https://doi.org/10.1016/j.jid.2022.09.413